litaba

Li-alkaloid tsa Toluidine tse joalo ka anisodamine le cyperine li na le lits'ebetso tsa bohlokoa tsa bioloji le boleng ba bongaka ba bongaka. Li-alkaloids tsena li fumanoa haholo-holo ka mekhoa ea khale ea ho tlosa limela tsa mantlha, le theknoloji ea Microbial heterologous synthesis e thehiloeng ho biology ea tlhaho e fana ka leano le lecha la ho hlophisoa ha bongaka ba tlhaho lihlahisoa.Ka biosynthesis ea n-methylpyrrolidine, l-ornithine e ne e sebelisoa e le selelekela sa pele, se ileng sa ts'oaroa ke ornithine.

safds

Bakeng sa ho theha lisele tse nang le heterologous le syntheine ea N - methyl pyrroline chassis XiaoYouLi sehlopha se llata sethaleng le xiao-dong li zhou sehlopha sa lipatlisiso, bafuputsi ba ile ba etsa lipatlisiso tse latelang: 1) karolo ea pele ea mohloli oa semela sa bongaka lintlha tse tharo tsa AaDAO2 amine oxidase le Ts'ebetso ea AaDAO3 ea biochemical, mohloli le ho kopanya lilekane tsa coca ornithine decarboxylase EcODC le mehloli ea putrescine ea anisodus tanguticus - N - methyl transase ho pitsa in vitro enzyme catalysis synthesis and screening of EcODC, AtPMT and AaDAO3 in vitro synthesis of N - methyl pyrroline modypase modimaline. ; 2) mefuta e meraro ea sebopeho mo mojuleng ona e ile ea kenngoa ka ho eketsehileng ka escherichia coli le saccharomyces cerevisiae ho theha lisele tsa chassis tse tsamaellanang le ho etsa tlhahlobo ea ho sisinyeha ha matla. N-methylpyrrolium e lebisitsoeng e fumanoe ka katleho ka lihlahisoa tsa 3.02 le 2.07mg / L ka ho latellana.3) motheong oa sena, bakeng sa ho eketsa tlhahiso, phuputso e boetse e kokota ho kenyeletsa chassis ea chastis ea N - methyl pyrroline tlholisano ea metabolic metabolism e amanang le enzymes ALD4, ALD5 le HFD1, ka nako e ts'oanang e hlahisa SAM2 e matlafalitsoeng ka enfelopole ea synthetic AtPMT e thusang ho kopanya li-cofactors tse hlokahalang bakeng sa SAM, qetellong li-chastis tsa chastis ea N - methyl pyrroline e fihletse 17.82 mg / L, bapisoa le khatello ea mantlha e eketsehile makhetlo a 8.6.

Boithuto bona e bile ba pele ba ho etsa sele ea microbial chassis bakeng sa heterologous synthesis ea n-methylpyrrolidine, e ileng ea rala motheo oa bohlokoa bakeng sa tlhahlobo le heterologous synthesis ea meriana alkaloids ea pele ho n-methylpyrrolidine.

Patlisiso eo e ile ea tšehetsoa hape ke yunivesithi ea zhejiang ea moprofesa oa bongaka ba setso ba China, zhang chuanyin, mme e tšehelitsoe ka chelete ke Setsi sa Mahlale sa China.Letshwao la profiles tsa metabolite le molek'hule e nyane NMR e ts'ehelitsoe ke sethala sa tšebelisano-leseling sa protomics le protheine ea setsi bakeng sa lits'ebeletso tsa mahlale a sechaba. setsi sa botle ba semela sa molek'hule


Nako ea poso: Mots'eanong-28-2020